Nucellar Embryogenesis and Artificial Seed Production in Citrus reticulata.
M. Sheshu Madhav
Department of Biotechnology, Assam Agricultural University, Jorhat, Assam No. 66 Hemanth, Indian Agricultural Research Institute, PUSA, Newdelhi-110 012, India.
S. Singh
Department of Biotechnology, Assam Agricultural University, Jorhat, Assam No. 66 Hemanth, Indian Agricultural Research Institute, PUSA, Newdelhi-110 012, India.
P. C. Deka
Department of Biotechnology, Assam Agricultural University, Jorhat, Assam No. 66 Hemanth, Indian Agricultural Research Institute, PUSA, Newdelhi-110 012, India.
B. N. Rao
Department of Biotechnology, Assam Agricultural University, Jorhat, Assam No. 66 Hemanth, Indian Agricultural Research Institute, PUSA, Newdelhi-110 012, India.
*Author to whom correspondence should be addressed.
Abstract
A study was undertaken to develop a protocol for nucellar embryogenesis and artificial seed preparation in Khasimandarin. Nucellar embryoids were formed in 35 days after culturing in NE4 media containing MT basal with malt extract (500mg l-1) (W/V)BAP( 0.5 mg l-1). These nucellar embryoids were used in preparation of artificial seed. Upon reculture, the artificial seed differentiated in to whole plants in 100-125 days in modified MT media containing in Malt extract (500 mg l-1) and BAP (0.25 mg l-1). Storage observations revealed that artificial seeds can be stored at lower temp (4 C) without altering their viability.
Keywords: Artificial seed, Embryoid, Nucelllar, Khasimandarin