In Vitro Propagation of Drosera indica L. An Endangered Insectivorous Plant
K. Nalini
Dalmia Centre for Research and Development, 9/369, Siruvani Main Road Kalampalayam, Coimbatore-641 010, India.
P. M. Murali
Dalmia Centre for Research and Development, 9/369, Siruvani Main Road Kalampalayam, Coimbatore-641 010, India.
*Author to whom correspondence should be addressed.
Abstract
Drosera indica L. is a threatened and endangered insectivorous plant. While there are successful tissue-culture protocols for a number of Drosera species, this is the first successful protocol for D. indica L. using stem segments as explants. Multiple shoots of D. indica was obtained in MS media supplemented with 5x10-7M 6-benzyl-amino purine and 2 x 10-7M napthaleneacetic acid. Rooting was obtained in 2 x 10-7M a-Napthoxyacetic acid. Under controlled conditions, the plantlets were successfully hardened. Re-introduction of plantlets back into their natural environment in the forests is presently underway.
Keywords: Droceraceae, Callus, Endangered, Insectivorous plant, Plumbagin, Tissue culture