Altered Segregation Ratio in Anther Culture derived Plant Progenies Generated from F1 Hybrids of Brassica juncea
Amitava Roy
Plant Tissue Culture Laboratory, The Agri-Horticultural Society of India 1, Alipur Road, Kolkata-700 027, India
P.K. Saha
Department of Botany, Bose Institute, 93/1, A. P. C. Road, Kolkata- 700 009
*Author to whom correspondence should be addressed.
Abstract
Microspore derived plant population was raised using anthers from F1 hybrid plants obtained by a reciprocal cross between two cultivars of Brassica juncea (cv. RM-198 and cv. RJ-15). The F2 population segregation ratio and frequency distribution of the hybrids were compared with the progenies of the microspore derived plant population (A2). It was observed that though F2 population showed simple ratio, non-Mendelian segregation was observed in A2 plants. Quantitative characters showed shift in mean and altered dispersion in A2 progenies in comparison to sexual hybrids.
Keywords: Brassica juncea, Hybrids, Microspore, Regeneration, Segregation ratio