High Frequency Plantlet Regeneration from Hypocotyl Explants of Tomato (Lycopersicon esculentum Mill.) via Organogenesis
P. Venkatachalam
Biotechnology Division, Rubber Research Institute of India, Kottayam-686 009, India.
N. Geetha
Plant Physiology Division, Rubber Research Institute of India, Kottayam-686 009, India.
P. Priya
Biotechnology Division, Rubber Research Institute of India, Kottayam-686 009, India.
G. Rajasegar
Department of Plant Science, Bharathidasan University, Tiruchirappalli-620 024, India
N. Jayabalan
Department of Plant Science, Bharathidasan University, Tiruchirappalli-620 024, India
*Author to whom correspondence should be addressed.
Abstract
A protocol was developed for high frequency plant regeneration from hypocotyls explants of Lycopersicon esculentumonMS medium supplemented with different concentrations and combinations of various auxins and cytokinins. Frequency of callus initiation and shoot bud regeneration varied with hormonal combinations and does in the medium. Among the combinations used, NAA (1.0 mg/l) and kinetin (0.1 mg/l) combination was found to be best for highest frequency of callus induction. Of the two cytokinins tested, BAP was found to be most suitable for maximum percentage of shoot bud differentiation as well as multiple shoot induction. Rooting of the regenerated shoots readily induced on half-strength MS medium supplemented with IBA. Well-developed plantlets were transferred to the field and 85% of them subsequently survived, flowered normally and set viable seeds.
Keywords: Hypocotyl explants, Lycopersicon esculentum, Plant regeneration, Plant growth regulators, Tomato