Identification of a Molecular Marker Linked to a Gene Conferring Resistance to Indian Pathotype- I Of Xanthomonas oryzae Pv Oryzae in Rice
J.S.S. Ammiraju
Department of Genetics, Osmania University, Hyderabad- 500 007, India, Biotechnology Center, Mahyco research Foundation, Hyderabad, India (K K J), Directorate of Rice Research, Rajendernagar, Hyderabad, India
K.K. Jena
Department of Genetics, Osmania University, Hyderabad- 500 007, India, Biotechnology Center, Mahyco research Foundation, Hyderabad, India (K K J), Directorate of Rice Research, Rajendernagar, Hyderabad, India
A.P.K. Reddy
Department of Genetics, Osmania University, Hyderabad- 500 007, India, Biotechnology Center, Mahyco research Foundation, Hyderabad, India (K K J), Directorate of Rice Research, Rajendernagar, Hyderabad, India
T. Diwaker
Department of Genetics, Osmania University, Hyderabad- 500 007, India, Biotechnology Center, Mahyco research Foundation, Hyderabad, India (K K J), Directorate of Rice Research, Rajendernagar, Hyderabad, India
K. Vaidyanath
Department of Genetics, Osmania University, Hyderabad- 500 007, India, Biotechnology Center, Mahyco research Foundation, Hyderabad, India (K K J), Directorate of Rice Research, Rajendernagar, Hyderabad, India
*Author to whom correspondence should be addressed.
Abstract
Bacterial Blight (BB) of rice is a major production constraint to rice farming in India. The feasibility of identifying molecular marker(s) linked to a bacterial blight resistance gene from a rice variety Ajaya. Which is non-allelic to other known recessive genes, conferring resistance to Indian pathotype I of Xanthomonas oryzae pv oryzae (Xoo) was investigated utilizing Random Amplified Polymorphic DNA (RAPD) technology. Bulk segregant analysis in conjunction with RAPD markers was used to identify DNA markers associated with bacterial blight resistance. With OPA-17 primer, a 485 bp polymorphic band was amplified only in resistant lines not in susceptible lines. This polymorphic band was found to be closely linked to the BB locus against Indian pathotype I.
Keywords: Bacterial blight, RAPD analysis, Bulked segregant analysis, Xanthomonas oryzea