Determination of Genetic Variability among Isolates of Tilletia indica using Random Amplified Polymorphic DNA Analysis
Avinash Mishra
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G. B. Pant university of Agriculture and Technology, Pantnagar- 263 145, India
Anil Kumar
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G. B. Pant university of Agriculture and Technology, Pantnagar- 263 145, India
G.K. Garg
Department of Plant Breeding, Punjab Agricultural University, Ludhiana, India
ndu Sharma
Department of Plant Breeding, Punjab Agricultural University, Ludhiana, India
*Author to whom correspondence should be addressed.
Abstract
Abstract: The polymerase chain reaction (PCR) in conjunction with six short arbitrary primers of random sequences was used to determine genetic variability in Karnal bunt pathogen. Total fifteen fungal isolates of Tilletia indica, collected from different regions of Punjab, were subjected to random amplified polymorphic DNA (RAPD) analysis. Among 15 fungal isolates used, consistent genetic relationship was generated by UPGMA cluster analysis. The RAPD profiles of 15 fungal isolates yielded 110 different RAPD markers, which categorized the fungal isolates into four RAPD groups (A1, A2, A3 and A4) with 17-35% similarity. Group A1 contained 9 isolates showing 27-35% similarity within group. Group A2 and A4 contained single isolate and group A3 showed 23-30% similarity amongst pathogen comprising 4 isolates.
Keywords: Tilletia indica, RAPD, Fungal isolates, Genetic relationship, Polymorphism