Plant Regeneration and In Vitro Flowering from Nodal Explants of Hydrolea zeylanica - A Rare Medicinal Plant
S. Ravi *
PG & Research Department of Botany, National College Tiruchirappalli-620 001, Tamil Nadu
B. Muthukumar
PG & Research Department of Botany, National College Tiruchirappalli-620 001, Tamil Nadu
E. Natarajan
PG & Research Department of Botany, National College Tiruchirappalli-620 001, Tamil Nadu
N. Ramesh Kannan
PG & Research Department of Botany, National College Tiruchirappalli-620 001, Tamil Nadu
K.R. Vijayalatha
Department of Horticulture, Rice Research Station, Tirurkuppam Tirur-602 025, Tiruvallur Dt, Tamil Nadu
*Author to whom correspondence should be addressed.
Abstract
An efficient micropropagation and in vitro flowering protocol was developed for an important and rare medicinal plant Hydrolea zeylanica. Multiple shoots regeneration from nodal explants was achieved on MS medium containing different concentration of BAP and Kinetin in combination with NAA. In vitro flowering was also observed after 8 days of shoot bud regeneration. Regenerated shoots rooted on MS medium fortified with different concentrations of IBA.
Keywords: Hydrolea, Microprogation, In vitro flowering, IBA