In vitro Plant Regeneration via Direct Organogenesis from Shoot Tip Explants of Cucumber (Cucumis sativus L. Green long)
P. Sangeetha
Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology Periyar University, Periyar Palkalai Nagar, Salem-636 011.
P. Venkatachalam *
Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology Periyar University, Periyar Palkalai Nagar, Salem-636 011.
*Author to whom correspondence should be addressed.
Abstract
The present study is focused on induction of multiple shoots from shoot tip explants of cucumber. Shoot tip explants were excised from 7-day-old seedlings and cultured on MS medium fortified with different concentrations of (0.5-2.5 mg/l) BAP alone and/or in combination with 0.5 mg/l KIN for shoot induction. The highest number of multiple shoot regeneration was noticed on MS medium supplemented with 1.0 mg/l BAP produced maximum frequency (89.8 %) with 9.8 shoots/culture. The elongated shoots were excised and cultured on half- strength MS medium containing different concentrations of (0.5-2.5 mg/l) IBA (Indole 3- butyric acid) and (0.5-2.5 mg/l) NAA a-Naphthalene acetic acid) alone and in combination with 0.5 mg/l KIN for rooting. Among the combinations used, IBA (1.5 mg/l) and KIN (0.5 mg/l) combination induced maximum percent of rooting (96.82%) with 8.24 roots/shoot. Well developed plantlets were successfully transferred into plastic cups containing a mixture of soil and sand in the ratio 1:1 and subsequently established in the green house and acclimatized in the field.
Keywords: Multiple shoot regeneration, Cucumis sativus L.,, 6-Benzyl aminopurine, Indole 3- butyric acid