In Vitro Micropropagation of Tylophora indica (Burm.F) Merril Through Shoot Tip Explants
T. Sellathurai
Department of Botany, Periyar University, Salem-636 011, Tamil Nadu, India
S. Rathinavel *
Department of Botany, Saraswathi Narayanan College (Autonomus), Madurai-625 022, Tamil Nadu
*Author to whom correspondence should be addressed.
Abstract
Clonal propagation of Tylophora indica from shoot tip explants placed on MS medium with low concentration of BAP (1 �M) and in combination with Ads (3 �M) induced three shoots and four roots. Explants on MS with BAP (4 �M) and KIN (3 �M) with Ads (4 �M) had less number of shoot and root. The responses of shoot tip explants in MS +2,4-D (12.5 �M) showed poor organogenesis or callusing. It may be concluded that further standardization of hormonal combinations could helpful to rapid propagation for conservation and commercial application.
Keywords: Tylophora indica, Shoot tip explants, MS medium, BAP, Micropropagation, Shoot multiplication