Development of an Efficient Protocol for High Frequency Plant Regeneration from Nodal Explants of Solanum nigrum L.
D. Mahendran
PG & Research Department of Botany, National College Tiruchirappalli, TN, India
S. Srinivasan
PG & Research Department of Botany, National College Tiruchirappalli, TN, India
M. Thiyagarajan
Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology Periyar University Salem-11, TN, India
P. Venkatachalam *
Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology Periyar University Salem-11, TN, India
*Author to whom correspondence should be addressed.
Abstract
An efficient protocol was devised for rapid propagation of Solanum nigrum L. from nodal explants. The nodal explants were cultured on MS medium supplemented with different concentrations (0.5-2.5 mg/l) of BAP and KIN for Multiple shoot bud induction. The highest rate of multiple shoot proliferation was observed on MS medium fortified with BAP (2.0 mg/l) followed by KIN (1.5 mg/l). The adventitious shoots were rooted successively on half strength MS medium supplemented with different concentrations of IBA (0.5-2.5 mg/l). The best rooting response was observed on 1 mg/l IBA. Regenerated rooted plantlets were successfully transferred into plastic cup containing a mixture of soil and sand in the ratio of 1:1. The 90% of the in vitro propagated plantlets survived when they were transferred to ex vitro conditions for acclimatization.
Keywords: Solanum nigrum L, MS medium, Nodal explants, Shoot multiplication