In Vitro Studies and RAPD analysis in Hybanthus enneaspermus (L.) F. Muell. - A Medicinal Plant
N. Ramesh Kannan
PG & Research Department of Botany, National College Tiruchirappalli-620 0001, Tamil Nadu
K.R. Vijayalatha
Department of Horticulture, Rice Research Station, Tirurkuppam, Tirur-602025 Tiruvallur District, Tamil Nadu
E. Natarajan *
PG & Research Department of Botany, National College Tiruchirappalli-620 0001, Tamil Nadu
*Author to whom correspondence should be addressed.
Abstract
Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of micropropagated plants of Hybanthus enneaspermus(L.) F. Muell. Ten arbitrary decamers were used to amplify DNA from in vivo and in vitro plant material to assess the genetic fidelity. All RAPD profiles from micropropagated plants were monomorphic and similar to those of field grown control plants. A variation was detected within the micropropagated plants. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the systems of micropropagation is discussed.
Keywords: Genetic Stability, Genetic markers, RAPD markers, Somaclonal variation, Micropropagation, Hybanthus enneaspermus