In vitro Regeneration of Alpinia galanga - An Important Medicinal Plant
S. Thriveni
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
S. Shashidhara
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
P.E. Rajashekharan
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
N. Deepak
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
Hanumantharaju
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
C.E. Rajendra
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
Dattatraya B Gawli
Department of Pharmacognosy, Government College of Pharmacy, Bangalore; Indian Institute of Horticulture Research, Bangalore, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Alpinia galanga comonly known as greater galangal is a tall, perennial, rhizomatous herb belongs to Zingiberaceae family. It is widely used in traditional system of medicine and it is facing threat of extinction owing to indiscriminate and unsustainable harvesting in the wild, Hence an in vitro regeneration method was developed for initiation and multiplication using rhizome explant. Murashige and Skoog (MS) medium supplemented with 0.1 mg/l NAA and 1.0 mg/l BAP induced maximum number of shoots and roots simultaneously. Regenerated plantlets were established initially in glass house and then transferred to garden soil, significant leaf expansion and leaf proliferation with root proliferation was observed which were uniform and identical to parent plant.