In vitro Regeneration from Leaf Callus Cultures of Scoparia dulcis
M. Jothi Basu *
Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India
A. Kalidoss
Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India
Kayathri
Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India
Shanmugan
Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India
S. Baburaj
Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India
*Author to whom correspondence should be addressed.
Abstract
Leaf explants of Scoparia dulcis L differentiated shoot buds from the callus on Murashige and Skoog s (MS) medium containing different combinations and concentrations of auxins and cytokinins or cytokinins alone. Shoot buds were formed either directly from the explants or from the callus. Regenerated shoots were successfully rooted on a medium containing half-strength MS and 2mg/l IAA. The regenerated plants were transferred to green house conditions with 75 to 80% of survival.
Keywords: Plant regeneration, Leaf callus, Scoparia dulcis, Medicinal herb