Role of Growth Regulators in Callus Establishment and Differentiation in Pluchea lanceolata (Dc.) C.B. Clarke
Deepika Arya
Plant Pathology, Tissue Culture and Biotechnology Laboratory, Department of Botany University of Rajasthan, Jaipur-302004, India
Neha Grover
Plant Pathology, Tissue Culture and Biotechnology Laboratory, Department of Botany University of Rajasthan, Jaipur-302004, India
Vidya Patni *
Plant Pathology, Tissue Culture and Biotechnology Laboratory, Department of Botany University of Rajasthan, Jaipur-302004, India
*Author to whom correspondence should be addressed.
Abstract
A protocol for callus establishment and differentiation in medicinally potent plant, Pluchea lanceolata has been developed by regulating the auxin-cytokinin ratio in basal media, source of explants and physiological state of the tissue in culture. Among the various explants (leaf/shoot tip/nodal segment) tried, leaf explants proved to be the best. The ideal medium for callus induction and establishment was MS medium supplemented with NAA (1.0 mg/l) in combination with BAP (0.5 mg/l). Maximum number of shoot buds (36.6 + 0.67) through callus differentiation was achieved on MS medium supplemented with BAP (1.0 mg/l) and Kn (1.0 mg/l). Elongation and further development of shoot buds into shoots (30.0 + 0.40) was achieved on MS medium fortified with BAP (0.25 mg/l) and Kn (0.5 mg/l). Histological study of callus showed the unorganized, loosely arranged, parenchymatous mass of cells while regenerable callus showed well-arranged shoot apices and leaf primordia. The intensity of peroxidase isozymes was appreciably higher in in vitro tissue (non-differentiating callus) as compared to in vivo tissues.
Keywords: Pluchea lanceolata, Callus differentiation, Histology, Isoperoxidase