In vitro Propagation of Arachis hypogaea L Var. Ak 1224: Direct and Indirect Plant Regeneration
Pintu Banerjee
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Visva-Bharati University Santiniketan � 731235, India.
Sharmistha Maity
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Visva-Bharati University Santiniketan � 731235, India.
Nirmalya Banerjee
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Visva-Bharati University Santiniketan � 731235, India.
*Author to whom correspondence should be addressed.
Abstract
In vitro propagation of Arachis hypogaea L through direct and indirect regeneration of shoots using various explants has been achieved. Cotyledonary nodes and cotyledonary leaves from axenic seedlings were cultured in presence of various concentrations (1-50 mg/l) of N6-benzylaminopurine (BAP) or kinetin (KIN). The frequency and number of regenerated shoots, axillary branches and shoot buds were higher in cotyledonary node compared to cotyledonary leaf culture. Small pieces of immature apical leaflets and nodal segments were cultured in presence of ?-napthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5-2.0 mg l-1) and BAP (0.5-2.0 mg/l) to induce callus. Nodal segment derived calli exhibited higher frequency of shoot regeneration compared to immature apical leaflet derived calli. Combined application of BAP (2.0 mg/l) and NAA (1.0 mg/l) gave optimum response in terms of indirect shoot regeneration. Regenerated shoots were rooted in presence of either 2,4-D (0.5 mg l-1) or NAA (1.0 mg /l) Well-rooted plantlets were successfully transferred to soil.
Keywords: Arachis hypogaea, Callus, In vitro regeneration, Organogenesis