Discrimination of Blue and White Flowered Clitoria ternatea Linn. (Fabaceae) Through RAPD assay
S. Wilson *
Centre for Biodiversity and Biotechnology, St.Xavier s College (Autonomous) Palayamkottai-627 002, Tamil Nadu, India
V.S. Manickam
Centre for Biodiversity and Biotechnology, St.Xavier s College (Autonomous) Palayamkottai-627 002, Tamil Nadu, India
Y. Justin Koilpillai
Centre for Biodiversity and Biotechnology, St.Xavier s College (Autonomous) Palayamkottai-627 002, Tamil Nadu, India
A. Victoria
Centre for Biodiversity and Biotechnology, St.Xavier s College (Autonomous) Palayamkottai-627 002, Tamil Nadu, India
S. Seeni
Centre for Biodiversity and Biotechnology, St.Xavier s College (Autonomous) Palayamkottai-627 002, Tamil Nadu, India
*Author to whom correspondence should be addressed.
Abstract
RAPD finger printing was used to measure the relatedness between the populations. RAPD primers were screened on a pool of genomic DNA of 16 samples of Clitoria ternatea bearing blue and white flowers. For each (both blue and white) variety, the DNA extracted from 4 individual from within the population (intra). The polymorphic amplified fragments were observed for 5 primers namely OPB - 11, 12, 17, 18 and 20 with high reproducibility. The percentage of polymorphism recorded was 48.1%. The genetic similarity matrix for inter and intra variation was calculated by primer wise comparison of 4 individuals of each blue and white populations.
Keywords: RAPD analysis, Clitoria ternatea, PCR amplification, Polymorphic band, DNA sample