Isolation of Cell Lines Resistant to Erysiphae cichoracearum by Screening Different Genotypes of Cucumis sativus L.
K. Venkateshwarlu
Department of Botany, Kakatiya University, Warangal, Andhra Pradesh-506 009, India
T. Shasthree *
Department of Botany, Kakatiya University, Warangal, Andhra Pradesh-506 009, India
A. Srinivas
Department of Botany, Kakatiya University, Warangal, Andhra Pradesh-506 009, India
B. Mallaiah
Department of Botany, Kakatiya University, Warangal, Andhra Pradesh-506 009, India
*Author to whom correspondence should be addressed.
Abstract
Leaf derived calli of four genotypes of cucumber susceptible, moderately resistant and resistant plants were screened in vitro for the production of powdery mildew disease (Erysiphae cichoracearum). Three methods were used to get resistance i.e., direct spore suspention, high (30%) and step-wise increase (10%, 20%, 30%) in culture filtrate concentration of Erysiphae cichoracearum. After being exposed to spore suspension, the susceptibility of calli were noticed and arrested the growth of the callus. However, certain parts of the calli were observed that were free from fungal attack and seen proliferating in the toxin containing media. Whereas, the susceptible calli turned brown resulting in death of the calli. Then the resistant cell lines were isolated and sub cultured for the three passages of two weeks. The percentage of these resistant cell lines varied among the four genotypes.
Keywords: Cell line, powdery mildew, Disease resistant, Disease resistant clones, Spore suspension, Stepwise selection, Culture filtrates, Resistant calli