Plant Regeneration from Nodal Segments in Phyllanthus amarus Schum and - An Important Medicinal Plant
R.S. Pagare
Department of Botany, University of Pune, Pune -411 007 (MS), India
R.B. Barmukh
Modern College, Shivajinagar, Pune- 411 007 (MS), India
K.M. Nitnaware
Department of Botany, University of Pune, Pune -411 007 (MS), India
T.D. Nikam *
Department of Botany, University of Pune, Pune -411 007 (MS), India
*Author to whom correspondence should be addressed.
Abstract
A plant regeneration protocol developed for Phyllanthus amarus Schum and Thonn; it is an important major raw material for Chavanprash (a worldwide used ayurvedic preparation) and a source of hepatoprotective chemicals phyllanthin and hypophyllanthin. The nodal explants were excised from naturally grown plants and aseptically cultured on MS medium supplemented with different concentration of auxins IAA, NAA and 2,4-D (0.0-22.0�M) and cytokinins BA and Kin (0.0-22.0�M), individually and in combinations. Among these, fortification of BA alone at 10 �M was effective for inducing maximum shoots (12.1�0.6) in nodal explants. The best condition for rooting of shoots was MS medium fortified with 7.5 �M IAA. After rooting, plantlets were transferred to earthen pots containing garden soil where 80-85% plantlets grew well and exhibited normal development.
Keywords: Phyllanthus amarus, Hepatoprotective, Micropropagation, Nodal explant