Direct Organogenesis from Leaf Explant of Solanum Burm F.
G.V. Priya
Department of Biotechnology, Kurinji College of Arts and Science, Trichy; Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore; PG and Research Department of Botany National College, Trichy-620 001
K.R. Vijayalatha
Department of Biotechnology, Kurinji College of Arts and Science, Trichy; Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore; PG and Research Department of Botany National College, Trichy-620 001
R. Sasikala
Department of Biotechnology, Kurinji College of Arts and Science, Trichy; Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore; PG and Research Department of Botany National College, Trichy-620 001
B. Muthukumar
Department of Biotechnology, Kurinji College of Arts and Science, Trichy; Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore; PG and Research Department of Botany National College, Trichy-620 001
E. Natarajan
Department of Biotechnology, Kurinji College of Arts and Science, Trichy; Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore; PG and Research Department of Botany National College, Trichy-620 001
*Author to whom correspondence should be addressed.
Abstract
A rapid in vitro method for propagation of Solanum surattense Burm. F. was developed. Leaf explants were cultured on MS medium supplemented with different concentration of BAP (4.44 � 17.76 �M/l) and KIN (4.65 - 18.60 �M/l) in combination with NAA (2.69 �M/l). Shoot buds initiated on the surface of the explants after 10 days of inoculation. The number of shoots was increased in subsequent subcultures. The well developed shoots were excised and rooted on MS medium supplemented with IBA (4.90 � 14.70 �M/l). All the regenerated plants were successfully acclimatized in natural environment.
Keywords: Solanum surattense, Micropropagation, Conservation of plants, Direct organogenesis, Plant hormones, Shoot buds