RAPD based Genetic Diversity analysis in Banana Hybrids
P.S. Kavitha *
Department of Fruit crops, Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
T.N. Balamohan
Department of Fruit crops, Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
B. Senthamizhselvi
Department of Fruit crops, Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
*Author to whom correspondence should be addressed.
Abstract
A total of 10 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers in phase I hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their pedigree. Out of the 10 primers studied, all the primers exhibited scorable markers. However, the maximum number of loci amplified was 18 with primer OPC 19 and the maximum polymorphism percentage was obtained with the primer OPF 2 and OPF 7.
Keywords: Phase I hybrids, Banana, Cluster analysis, DNA polymorphism, Genetic diversity, RAPD