In Vitro Plant Regeneration from Shoot Meristem Explants of Wheat (Triticum aestivum L.)
Vanita Jain *
Division of Plant Physiology, Indian Agricultural Research Institute New Delhi-110 012
Sangeeta Khetarpal
Division of Plant Physiology, Indian Agricultural Research Institute New Delhi-110 012
Smitha Aravind
Division of Plant Physiology, Indian Agricultural Research Institute New Delhi-110 012
P. Ananda Kumar
NRC for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110 012
G. C. Srivastava
Division of Plant Physiology, Indian Agricultural Research Institute New Delhi-110 012
*Author to whom correspondence should be addressed.
Abstract
A simple procedure for high frequency multiple shoot regeneration from shoot meristem of wheat (Triticum aestivum L.) has been developed. Shoot apices from 1-week-old aseptically grown seedlings were cultured in vitro on MS medium supplemented with either Benzylamino purine (BA, 2 mg/l) or Thidiazuron (TDZ, 2 uM and 5 uM/l). Both BAP and TDZ induced the production of multiple shoots from enlarged apical meristems. Fertile wheat plants were produced from in vitro regenerated multiple shoots.
Keywords: Multiple shoot induction, Regeneration, Shoot meristem, Wheat