In vitro shoot Regeneration from Leaf Explants of Vitis vinifera (L.)
U.S. Kurmi
Horticultural Biotechnology Laboratory[U.S.K.,D.K.S.,M.K.T.],Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
D.K. Sharma
Horticultural Biotechnology Laboratory[U.S.K.,D.K.S.,M.K.T.],Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
M.K. Tripathi
Horticultural Biotechnology Laboratory[U.S.K.,D.K.S.,M.K.T.],
R. Tiwari
Department of Fruit Science[R.T.],Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
S.N Mishra
Department of Soil Science[S.N.M.],Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
B.S. Baghel
Office of Dean, KNK-College of Horticulture[B.S.B.],Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
S. Tiwari
Mandsaur[S.T.], Division of Transgenic and Tissue Culture, Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
*Author to whom correspondence should be addressed.
Abstract
An in vitro regeneration protocol has been developed from 5-8 mm long leaf discs excised from in vitro grown regenerants as well as one-year-old plants of three different cultivars of Vitis vinifera L viz: Thompson seedless, Karnet and Local to find out the best in vitro responsive cultivar, culture media combination (especially appropriate auxin/cytokinin ratio) with their interactive effects on frequency of callus induction, formation of morphogenic calli and plantlet regeneration. MS medium fortified with 2.0-3.0 mg/l 2, 4-D and 0.5 mg/l Kinetin or BA proved more responsive for callus induction, while morphogenic calli as well as plantlets in higher frequencies were investigated with culture medium amended with 2.0 mg/l BA in conjunction with 0.5 mg/lNAA. In term of genotypic response, genotype Thompson Seedless responded better than Karnet and Local. Higher in vitro rooting response i.e. root proliferation ability, number (s) of roots and root length was exhibited by MS rooting medium fortified with 2.0 mg/lIBA and 15 g.l-1 sucrose. The in vitro raised plantlets were acclimatized and established successfully in the field.
Keywords: Vitis vinifera, leaf disc culture, morphogenesis, somatic embryogenesis, organogenesis, plantlet regeneration