Direct Regeneration in Glossocardia bosvallea: A Medicinal Plant
R. Geetha *
Department of Botany, Regional Institute of Education, Mysore- 570006, Karnataka, India.
G.V. Gopal
Department of Botany, Regional Institute of Education, Mysore- 570006, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
In vitro plantlets were directly regenerated from nodal explants of Glossocardia bosvallea (L.f) DC on Murashige and Skoog's (MS) medium supplemented with varied concentrations of BAP, NAA and KN. However, multiple shoots were generated from nodal explants of Glossocardia bosvallea on MS media supplemented with 2mg/l, 6- benzylamino purine. In vitro flower heads were also obtained in mature plantlets on the MS media supplemented with 1mg/l. NAA and 2mg/l KN. Individual shoots were excised and rooting was induced in vitro on MS medium supplemented with indole 3- butyric acid. Regenerants acclimated in soil rite showed vigorous shoot growth (within 9 weeks) and after 2-3 months was suitable for direct field planting.
Keywords: Multiple shoots, Nodal explants, Direct regeneration, In vitro flowering