In Vitro Plant Regeneration of Crape Myrtle : An Ornamental Shrub
M. H. Niranjan
Plant tissue culture lab, PG Department of Botany, Manasagangotri, Mysore-570 006, India
M.S. Sudarshana
Plant tissue culture lab, PG Department of Botany, Manasagangotri, Mysore-570 006, India.
Nayeemulla Shariff
Plant tissue culture lab, PG Department of Botany, Manasagangotri, Mysore-570 006, India
Dharmendra *
Plant tissue culture lab, PG Department of Botany, Manasagangotri, Mysore-570 006, India.
*Author to whom correspondence should be addressed.
Abstract
Efficient method for plant regeneration via organogenesis from leaf explants of crape myrtle (Lagerstroemia indica L.) has been developed. Leaf explants devoid of petiole cultured on solid ms medium supplemented with phytohormones, released phenolic exudates which adversely affected the culture response, the explants turned brown and ultimately necrosed. Addition of 100 mg/l ascorbic acid (AA) to ms medium checked the release of phenolic exudates and a little amount of callus was induced on different hormonal supplements. MS + 2,4-d (0.5 mg/l) + BA (1.0 mg/l) with AA (100 mg/l) proved effective for the induction of organogenic callus, on sub-culturing the callus on MS + 2,4-D (0.5 mg/l) + BA (1.0 mg/l) with AA (50 mg/l) multiple shoots differentiated. The micro shoots were rooted on ms liquid medium containing IBA at 1.0 mg/l. The regenerated plantlets were hardened and transferred to the garden soil.
Keywords: Crape myrtle, leaf explant, ornamental plant, regeneration