Production of Asiaticoside from Callus Cultures of Centella asiatica (L) Urban
Hasan Pasha Sholapur
Department of Pharmacognosy and Pharmaceutics, K.L.E.S� College of Pharmacy, Vidyanagar, Hubli-31
V.I. Hukkeri
Department of Pharmacognosy and Pharmaceutics, K.L.E.S� College of Pharmacy, Vidyanagar, Hubli-31
Fatima Sholapur Sholapur
Department of Pharmacognosy and Pharmaceutics, K.L.E.S� College of Pharmacy, Vidyanagar, Hubli-31
M.D. Altaf
Department of Pharmaceutics, R.M.E.S� College of Pharmacy Old javargi road, Gulburga
Imran Pasha *
Department of Pharmaceutics, R.M.E.S� College of Pharmacy Old javargi road, Gulburga
*Author to whom correspondence should be addressed.
Abstract
Centella asiatica (L.) Urban belonging to the family Apiaceae is a perennial herb with high medicinal values in traditional and modern medicine especially as brain tonic, anti-tubercular and anti-leprotic. The main objective of this study was to optimize the hormone concentration for callusing and to determine the yield of asiaticoside a compound previously isolated from whole plant of Centella asiatica. MS media supplemented with NAA (1 mg/l) and BAP (2 mg/l) was found to be suitable for the establishment of callus cultures from roots of Centella asiatica (L) Urban. The callus upon extraction and analysis revealed the presence of a triterpenoid saponin, asiaticoside 1.11�0.096 mg/g dry weight.
Keywords: Centella asiatica, Asiaticoside, Alkaloid production, Callus culture.