Effect of Colchicine on Callus Induction from Isolated Microspores in Hevea brasiliensis
R. Jayashree
Biotechnology Division, Rubber Research Institute of India, Kottayam- 686009, Kerala, India.
K. Rekha
Biotechnology Division, Rubber Research Institute of India, Kottayam- 686009, Kerala, India.
P. Kumari Jayasree
Biotechnology Division, Rubber Research Institute of India, Kottayam- 686009, Kerala, India.
R. Sanju
Biotechnology Division, Rubber Research Institute of India, Kottayam- 686009, Kerala, India.
A. Thulaseedharan *
Biotechnology Division, Rubber Research Institute of India, Kottayam- 686009, Kerala, India.
*Author to whom correspondence should be addressed.
Abstract
A method for the induction of haploid callus from isolated microspores of Hevea brasiliensis has been developed. The growth promoting effects of colchicine on callus formation was proved. Anthers at the late uni-nucleate to the early bi-nucleate stage of the microspores were pretreated at 4�C for 5-7 days before microspore isolation. Different durations of colchicine exposure (24-96 hrs) as well as varying concentrations (100-1000 mg/l) were tested after the pre-culture. The maximum response was noticed when the microspores were pre-cultured in 100 mg/l colchicine for 96 hrs. It was observed that a high sucrose concentration (180 g/l) favored callus growth in the presence of the growth regulators, kinetin and zeatin (3.0 mg/l). This is a major step towards utilization of haploid technology in Hevea breeding.
Keywords: Androgenesis, Colchicine, Hevea brasiliensis, Isolated microspores