Establishment of an Efficient Cell Suspension Culture in Acacia confusa (Merr.) For Induction of Antioxidants by Using Elicitors
S. Arumugam
School of Forestry and Resource conservation National Taiwan University [S.A., F.C., S.T.C.] Department of Forestry, National Chung-Hsing University Taichung 402, Taiwan
Fang-Hua Chu
School of Forestry and Resource conservation National Taiwan University [S.A., F.C., S.T.C.] Department of Forestry, National Chung-Hsing University Taichung 402, Taiwan
Sheng-Yang Wang
School of Forestry and Resource conservation National Taiwan University [S.A., F.C., S.T.C.] Department of Forestry, National Chung-Hsing University Taichung 402, Taiwan
Shang-Tzen Chang
School of Forestry and Resource conservation National Taiwan University [S.A., F.C., S.T.C.] Department of Forestry, National Chung-Hsing University Taichung 402, Taiwan
*Author to whom correspondence should be addressed.
Abstract
The current study is focused on the production of antioxidants in cell suspension culture of Acacia confusa (Merr.) by using various elicitors, including jasmonic acid (JA), jasmonic acid + benzylaminopurine (JA + BA), abscisic acid (ABA) and ultraviolet irradiation (UV) treatment. Friable calli were initiated on Murashige and Skoog (MS) solid medium containing 2,4-D 3 mg/l, and established cell suspension culture in MS liquid medium containing 2,4-D 2 mg/l + coconut milk 10%. The suspension cells then were treated with the elicitors. Among them the extracts produced from JA 5 mg/l + BA 6 mg/l treatment showed a significant inhibitory activity against the DPPH radical with increasing growth rate of suspension cells. The other three treatments, JA, ABA and UV-irradiation, however did not show the antioxidative activity against DPPH radical and reduction of cell suspension mass. In addition, the content of total phenolics was determined mainly by JA + BA treated cell suspension extracts. In addition, this study has identified cell viability, nature of callus, secondary callus formation and pH variation in medium. At this stage the result has shown that the integration of cytokinin (BA) with jasmonic acid might play an important key role to produce antioxidants in the cell suspension culture of A. confusa.
Keywords: Acacia confusa (Merr.), antioxidant activity, elicitor, callus, suspension cell culture