In Vitro Development and convertibility of Nucellar Embryos of Mangifera indica L. Bappakai, A Dwarfing Rootstock
H. C. Chaturvedi *
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
M. Sharma
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
S. Agnihotri
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
A. K. Sharma
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
M. Jain
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
A. Chourasia
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
N. R. Kidwai
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
P. Gupta
Tissue Culture Laboratory, National Botanical Research Institute, Lucknow- 226 001, India
*Author to whom correspondence should be addressed.
Abstract
Embryos differentiated fromm the nucellus of young ovular halves of Mangifera indica L. Bappakai, and important dwarfing rootstock variety, on BM1 medium supplemented with 0.25 mgl-1 BA and 1 mgl-1 NAA. Their proliferation was achieved in the medium containing 0.25 mgl-1 N6-(2-isopentenyl) adenine and 0.5 mgl NAA, a decline in magnitude of embryo proliferation noticed in long-term cultures. For near-synchronized development, maturation, germination (visible plumule with developed root) and convertibility (plantlet fornation) of cotyledonary embryos, the liquid state of nutrient medium was required. The presence of 0.01 mgl-1 abscisic acid together with 100 mgl-1 polyethylene glycol (M. Wt. 400) and 0.1 mgl-1 IAA in the basal medium BM2 promoted development and maturation of embryos followed by their germination. The maximum percentage of germination obtained was 94% with embryos of 1.5 cm and 2 cm in length. Howeverr, 80% of such germinated embryos converted into plantlets, with well-developed green leaves, on subculture in the same medium in the former case, while 76% in the latter. The in vitro-raised plantlets showed about 50% successs in harding.
Keywords: Near-synchronized development, Nucellar embryo proliferation, Ovular halves, Plantlet formation, Transplant success