Effect of Genotype and Culture Medium on Callus Proliferation and Morphogenesis of Immature Embryonic Axes of Soybean (Glycine Max L. Merr.)
Manoj Tripathi
Plant Biotechnology Laboratory, Biotechnology Centre Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
Sharad Tiwari *
Plant Biotechnology Laboratory, Biotechnology Centre Jawaharlal Nehru Agricultural University, Jabalpur 482 004, India
*Author to whom correspondence should be addressed.
Abstract
Immature embryos of eleven genotypes of Glycine max were cultured on MS medium supplemented with different levels of growth hormones, viz.8.0 mg.l-1 NAA (MS8N), 2.0mg.l-1 NAA + 0.2mg.l-1 BA (MS2NB) and 3.0 mg.l-1 BA + 0.5mg.l-1 NAA (MS3BN). Highly significant differences were noticed in the response of genotypes, culture media and genotype x medium interactions for callus initiation and plantlet regeneration. Results indicated that the combination of BA at higher level and NAA at lower level was found to be most responsive for tissue culture experiments of soybean.
Keywords: Soybean, Glycine max, Embryo culture, Embryogenesis