In Vitro Adventitious Shoot Formation from Leaf Explants of Withania somnifera Dunal.
I. Sivanesan *
Centre for Advance Studies in Botany, University of Madras, Guindy campus, Chennai- 600 025 Tamil Nadu, India; Best Biotech Research Labs (P) Ltd., #221, 2nd floor, 9th main, 5th Block Jayanagar, Bangalore- 560 041, Karnataka, India
K. Murugesan
Centre for Advance Studies in Botany, University of Madras, Guindy campus, Chennai- 600 025 Tamil Nadu, India; Best Biotech Research Labs (P) Ltd., #221, 2nd floor, 9th main, 5th Block Jayanagar, Bangalore- 560 041, Karnataka, India
*Author to whom correspondence should be addressed.
Abstract
An efficient protocol was developed for high frequency plant regeneration from leaf explants of Withania somnifera Dunal on Murashige and Skoog (1962) medium supplemented with different concentrations of Auxins and cytokinins. Frequency of shoot bud regeneration varied with dose of plant growth regulators in the medium. Highest frequency of shoot buds was obtained at a concentration of 1.0 mg/l Kin. In vitro rooting of microshoots were obtained by growing them in half strength MS medium supplemented with 2.0 mg/l IBA. Rooted plantlets were successfully transferred to the field, after acclimatization in the net house.
Keywords: Endangered plant, Withania somnifera, Direct egeneration, Leaf explants