In Vitro Proliferation of Hyptis suaveolens Poit: An Ethno-Medicinal Herb
Amruta Jain
Department of Botany, University Campus, Nagpur, Maharashtra
Alka Chaturvedi
Department of Botany, University Campus, Nagpur, Maharashtra
*Author to whom correspondence should be addressed.
Abstract
In the present study, plant regeneration was achieved via multiple shoots in Hyptis suaveolens. The apical buds of the three days old seedlings could be stimulated to undergo multiple shoot formation on Murashige and Skoog s medium supplemented with 1 mg/l BAP and 0.2 mg/l NAA. The multiple shoots then separated and cultured in Erlenmayer flasks containing same fresh medium. On the same media rhizogenesis was noticed after 15 days of culture initiation. The regenerated shoots yields essential oil as in the intact plant. The plants subsequently transferred to soil and kept in green house conditions.
Keywords: Apical buds, Hyptis suaveolens, Micropropagation