In Vitro Regeneration of Vitex negundo L., A Medicinal Shrub
Sweta Das *
School of Studies in Life Sciences, Pt. Ravishankar Shukla University Raipur- 492 010, Chhattisgarh, India
Vimal Kanungo
School of Studies in Life Sciences, Pt. Ravishankar Shukla University Raipur- 492 010, Chhattisgarh, India
M. L. Naik
School of Studies in Life Sciences, Pt. Ravishankar Shukla University Raipur- 492 010, Chhattisgarh, India
Sanju Sinha
School of Studies in Life Sciences, Pt. Ravishankar Shukla University Raipur- 492 010, Chhattisgarh, India
*Author to whom correspondence should be addressed.
Abstract
Vitex negundo commonly known as five- leaved chaste tree or Nirgundi is a deciduous shrub with thin grey bark. A successful protocol for in vitro regeneration of V. negundo from nodal explants on MS medium supplemented with different concentrations of BAP, NAA and Kn. Multiple shoots were developed on MS medium supplemented with 1 mg/L BAP. Roots were initiated within 15 days on half strength MS medium supplemented with 0.1 mg/L IBA. In vitro flower heads were also noticed in the matured plantlets in the same medium. The rooted plantlets were successfully established in the field.
Keywords: Vitex negundo, Tissue culture, Direct regeneration, Multiple shoot induction