Molecular Characterization Of Cocoa (Theobroma cocoa L.) Germplasm Using RAPD Markers
Anuradha Sane *
Central Plantation Crops Research Institute, Regional Station, Vittal-574 243, Karnataka, India and Central Horticultural Experiment Station, Hirehalli-572168, Tumkur, Karnataka
A. Chandra Sekar
Central Plantation Crops Research Institute, Kasaragod-671 124, Kerala, India
*Author to whom correspondence should be addressed.
Abstract
Forty Cocoa (Theobroma cacao L.) accessions obtained from Nigeria were characterized using 12 random oligonucleotide primers by RAPD analysis. The Jaccard�s similarity coefficient estimated from 139 RAPD markers obtained using 12 random primers. Cluster analysis using UPGMA method differentiated forty accessions into 3 clusters at 80% similarity level. Principal component analysis showed similar cluster pattern. Five accessions showed divergence.
Keywords: Oryza sativa, Rice, Plant regeneration, Plant transformation, Particle gun, Agrobacterium