Plant Regeneration from Mature Embryo Derived Calli of Triticum aestivum and Triticum durum
S. Tiwari *
Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics, Plant Tissue Culture and Genetic Transformation Division, Biotechnology Centre Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur 482004
D. K. Agrawal
Division of Crop Improvement, Central Institute for Cotton Research, Nagpur 440 010, India
M. K. Tripathi
Division of Crop Improvement, Central Institute for Cotton Research, Nagpur 440 010, India
R. S. Shukla
Division of Crop Improvement, Central Institute for Cotton Research, Nagpur 440 010, India
*Author to whom correspondence should be addressed.
Abstract
Mature embryos from 16 genotypes of Triticum aestivum and 4 genotypes of T. durum were cultured on MS medium with 4 modifications viz. MS2D (2.0 mg.1-1 2,4-D), MS 5D (5.0 mg.1-1 2,4-D), MSDC (5.0 mg.1-1 2,4-D + 100 ml.l-1 coconut milk) and MSDK (2.0 mg.1-1 2,4-D + 0.5 mg.1-1 kinetin). Considerable amount of variability was observed among genotypes, culture media and genotype x culture medium interactions. Embryos from the genotype JWJ 90-B-25 initiated maximum callus and exhibited maximum embryogenic callus formation. Medium MS2D responded maximum for these two traits; however, plant regeneration was lowest from callus cultures raised on this medium. Addition of coconut milk and 2,4-D to the basal MS medium (MS2D) regenerated maximum plantlets followed by MS5D, MSDK and MS2D.
Keywords: Mature embryo culture, genotype x medium interaction, Triticum aestivum, Triticum durum