Organogenesis and Plant Regeneration from Seedling Explants of Eucalyptus tereticornis
M. G. Prakash *
Institute of Forest Genetics and Tree Breeding, Coimbatore-641 002, Tamil Nadu, India
K. Gurumurthi
Institute of Forest Genetics and Tree Breeding, Coimbatore-641 002, Tamil Nadu, India
*Author to whom correspondence should be addressed.
Abstract
Adventitious shoot formation and plant regeneration was obtained from cotyledon and hypocotyls explants of Eucalyptus tereticornis Sm. Collected from aseptically grown seedlings. The explants were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of NAA (0-5 mg/l) and BAP (0-5 mg/l). Callus development and shoot morphogenesis occurred from all explants with varying degrees on different growth regulator concentrations tested. The shoot buds appeared emerging as dark pink colored protuberances on the callus. The multiplication, elongation and rooting of shoots were found to be best on hormone free basal medium. The rooted plantlets were established in pots after hardening.
Keywords: Cotyledons, Eucalyptus tereticornis, Hypocotyl, Plantlets, Shoot morphogenesis