Genetic Stability of In Vitro raised Sugarcane Plantlets by RAPD Markers
Sangeeta Srivastava *
Division of Crop Improvement Indian Institute of Sugarcane Research, Lucknow - 226002, India.
Radha Jain
Division of Plant Physiology and Biochemistry Indian Institute of Sugarcane Research, Lucknow - 226002, India
P. S. Gupta
Division of Crop Improvement Indian Institute of Sugarcane Research, Lucknow - 226002, India.
Jyotsna Singh
Division of Plant Physiology and Biochemistry Indian Institute of Sugarcane Research, Lucknow - 226002, India.
*Author to whom correspondence should be addressed.
Abstract
Micropropagated plantlets produced by shoot-tip culture of two varieties of sugarcane were evaluated for genetic stability using randomly amplified polymorphic DNA (RAPD) analysis. Distinct and reproducible RAPD markers obtained through eight decamer random primers gave almost monomorphic banding profile for both the genotypes except a few weak bands across all the plantlets analyzed. Thus, a total of 82 bands were generated, exhibiting homogeneous RAPD patterns for the plantlets tested. These results suggest that the in vitro culture conditions used for shoot-tip culture are appropriate for clonal propagation of sugarcane, as they do not seem to interfere with the genetic integrity of the regenerated plantlets.
Keywords: Genetic stability, RAPD markers, Micropropagation, Saccharum, Sugarcane, Shoot tip culture.