Micropropagation of Spilanthes paniculata Wall Ex. Dc.
G. Mahendran
Plant tissue culture Laboratory, Department of Botany, Bharathiar University, Coimbatore - 641 046.
K. Lavanya Devi
Plant tissue culture Laboratory, Department of Botany, Bharathiar University, Coimbatore - 641 046.
V. Narmatha Bai *
Plant tissue culture Laboratory, Department of Botany, Bharathiar University, Coimbatore - 641 046
*Author to whom correspondence should be addressed.
Abstract
An efficient protocol was developed for high frequency shoot regeneration from the nodal explants of Spilanthes paniculata. Murashige and Skoog (MS) medium supplemented with BAP (3mg/l) was found to be most effective in initiating multiple shoots at the rate of 35 per explant and BAP in combination with KIN (1mg/l) or NAA (0.5-1mg/l) also induced multiple shoots. Both shoot and root developed in the same medium. Microshoots rooted best in vitro, in half strength MS medium supplemented with (0.5mg/l) IAA (0.1mg/l) and NAA (0.1 mg/l). Regenerated plantlets were successfully established in soil with a survival rate of 95%.
Keywords: Spilanthes paniculata, Micropropagation, Medicinal plant, Nodalexplant, Multiple shoots, Spilanthol