Optimal PCR Parameters for RAPD Analysis in Theobroma cacao L. and Cola nitida
Anuradha Sane *
Central Plantation Crops Research Institute, Regional Station, Vittal, 574 243, Karnataka, India and Central Horticultural Experiment Station, Hirehalli 572 168, Tumkur, Karnataka
A. Chandrasekhar
Central Plantation Crops Research Institute, Kasaragod, Kerala
R. Manimekalai
Central Plantation Crops Research Institute, Kasaragod, Kerala
*Author to whom correspondence should be addressed.
Abstract
A reproducible PCR protocol is a prerequisite for usefulness of RAPD markers in germplasm analysis. To get optimum results, optimizing PCR protocol involving PCR parameters and reactions conditions is very essential. This paper discusses the optimization of different PCR parameters for getting reproducible results.
Keywords: Cola nitida, DNA, Optimization, PCR, RAPD, Theobroma cacao