In Vitro Selection of Pigeon Pea Cell Lines and Regeneration of Plantlets from Tolerant Callus to Culture Filtrate of Fusarium odum Buttler
Srinath Rao *
Plant Tissue Culture Laboratory, Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India.
K. Basavaraj
Plant Tissue Culture Laboratory, Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India.
C.P. Kaviraj
Plant Tissue Culture Laboratory, Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Fusarium odum causes severe wilt disease in Pigeon Pea (Cajanus cajan L.) Mill. The present study reports selection of calli tolerant to culture filtrate of Fusarium odum. Cotyledon calli were selected for their tolerance of F. odum culture filtrate (FCF). The increase of culture filtrate concentration has shown progressive browning and reduction of fresh and dry weight of callus. Plantlets were regenerated from FCF selected calli on MS medium supplemented with 1.0mg/l BAP+0.1 mg/l IAA. The plantlets obtained from the FCF selected calli showed tolerance to culture filtrate of Fusarium.
Keywords: Cajanus cajan, Fusarium odum, In vitro, Cell Line Selection