In Vitro Shoot Regeneration from Leaf Segments of Carnation (Dianthus Caryophyllus L.) Via Indirect Organogenesis
Ajit Kumar *
Department of Horticulture, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar-263 145,U.S. Nagar, Uttaranchal, India
Anju Verma
Division of Floriculture and Landscaping, National Research Centre on Plant Biotechnology
S.K. Singh
Division of Fruits and Horticultural Technology
S.P.S. Raghava
Indian Agricultural Research Institute, New Delhi � 110 012, India
P. A. Kumar
Indian Agricultural Research Institute, New Delhi � 110 012, India
*Author to whom correspondence should be addressed.
Abstract
Leaf segments were prepared and cultured on MS medium supplemented with different concentrations and combinations of growth regulators. Among the combinations tried, highest frequency of callus induction was obtained with 1.0 mg/l 2,4-D + 2.2 mg/l TDZ + 0.12 mg/l zeatin combination. Maximum frequency of shoots was noticed on MS medium containing 0.6 mg/l TDZ and 1.2 mg/l zeatin and also gave rise to 27.0 shoot buds per callus mass. The regenerated shoots were elongated on medium with 0.5 mg/l GA and rooted plants were then established in greenhouse for hardening.
Keywords: Callus induction, Leaf segment, Shoot bud induction, Thidiazuron