Possible Role of Receptor Like Kinase(S) in Karnal Bunt Pathogenesis and Resistance
Amit Anand
Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar- 263 145, Uttaranchal, India
G. K. Garg
Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar- 263 145, Uttaranchal, India.
Sneh Narwal
Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar- 263 145, Uttaranchal, India.
Anil Kumar *
Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar- 263 145, Uttaranchal, India.
*Author to whom correspondence should be addressed.
Abstract
Four sets of primers were selected to amplify the members of receptor-like kinase gene family. However, amplification was observed with only one set of primers and only in six out of 20 wheat genotypes tested. The set of primers that amplified 6 varieties was based on sequences of homologous genes of hexaploid and diploid wheat of European varieties (showed considerable polymorphism due to transposable elements) hence; all Indian varieties could not be amplified by PCR. This differential amplification among 20 wheat genotypes may be due to segregation and rearrangement events due to gene deployment during the varietal development. Moreover, results could not be linked definitely with the KB resistance phenomenon until a further study would be conducted.
Keywords: Karnal bunt, Pathogenesis, Polymerase chain reaction, Receptor-like kinase, Resistance