In Vitro Storage of Gladiolus: Use of Growth Retardants and Osmotica
M.S. Patil
Department of Plant Propagation and Biotechnology Kittur Rani Channamma college of Horticulture, Arabhavi-591 310 University of Agriculture Sciences, Dharwad, India
J. Dinakara Adiga *
Department of Plant Propagation and Biotechnology Kittur Rani Channamma college of Horticulture, Arabhavi-591 310 University of Agriculture Sciences, Dharwad, India
S.B. Kotimani
Department of Plant Propagation and Biotechnology Kittur Rani Channamma college of Horticulture, Arabhavi-591 310 University of Agriculture Sciences, Dharwad, India
Shankarnag
Department of Plant Propagation and Biotechnology Kittur Rani Channamma college of Horticulture, Arabhavi-591 310 University of Agriculture Sciences, Dharwad, India
*Author to whom correspondence should be addressed.
Abstract
The effect of alar, maleic hydrazide and mannitol on growth and storability of micro-shoots of gladiolus was assessed. Alar (B-9) at a concentration of 40 mg per litre was best in extending the storability of cultures with the least shoot length (7.20 cm) and highest percent recovery (92.00%). The same concentration (4.0mg per litre) of MH delayed the bud sprouting (26.80 days) with least number of multiple shoots (0.20), reduced shoot length (7.96 cm) and longest storage period (270.00 days). Mannitol at 2 percent was best in extending storage period (273.80 days) with good percent recovery (88.00%).
Keywords: Tissue culture, In vitro, Conservation, Alar, Maleic hydrazide, Mannitol