Plantlet Regeneration through Multiple Shoot Induction in Cajanus cajan (L.)
P. Balaramaswamy Yadav
Department of Botany, Andhra University, Visakhapatnam-530 003, India
V. Padmaja *
Department of Botany, Andhra University, Visakhapatnam-530 003, India
*Author to whom correspondence should be addressed.
Abstract
A simple protocol was established for plantlet development through multiple shoot induction, elongation and their rooting from the mature seed explants of Cajanus cajan (L.) Millspaugh cv.ICPL93115. Murashige and skoog (1962) medium supplemented with 6-benzyl adenine at 5-10mg-1 appeared to be adequate for inducing an average of 8-18 shoots per explants. However, increase in the number of shoots induced was associated with hindrance to their elongation.MS + BA (1.0mgl-1) promoted shoot elongation, while half strength MS medium with 1.0 mgl-1 of IAA/NAA + 0.1mgl-1 of KN facilitated rooting.
Keywords: Cajanus cajan, Multiple shoot induction, Pigeonpea, Plant regeneration