In Vitro Plant Regeneration from Nodal Explants of Syzygium cuminii L.
A. B. Remashree *
Biomass Research Project (MNES, Govt. of India) Department of Botany, University of Calicut,673 635, India
T. Varghese Thomas
Biomass Research Project (MNES, Govt. of India) Department of Botany, University of Calicut,673 635, India
E. Nabeesa
Biomass Research Project (MNES, Govt. of India) Department of Botany, University of Calicut,673 635, India
N. Neelakandan
Biomass Research Project (MNES, Govt. of India) Department of Botany, University of Calicut,673 635, India
S. Nandakumar
Biomass Research Project (MNES, Govt. of India) Department of Botany, University of Calicut,673 635, India
*Author to whom correspondence should be addressed.
Abstract
Callus induction and high frequency regeneration was obtained from nodal explants of five-year-old plants of Syzygiun cuminii when cultured on WP medium supplemented with 2,4-D (4.5 ?M) and BA (4.4 ?M). Calli were subcultured on WP medium with different concentrations of BA (0.04-17.7?M). Shoot initiation was observed in 4.4 ?M -13.3 ?M BA containing medium but addition of Kn (0.46-23.2 ?M) increased the number of shoots within 40-50 days. Rooting of shoots occurred in WP medium supplemented with IBA (0.24-4.9 ?M) or 1.5mg/l charcoal. Sixty to seventy percent of the hardened plants were survived after transfer to soil.
Keywords: Multipurpose tree, Nodal explant, Plant regeneration, Syzygium cuminii