In Vitro use of a Virus Inhibitor of Plant Origin in the Production of Carnation Latent Virus (Clv) Free Plants
Manisha Mangal
Department of Biotechnology Dr. Y S Parmar University of Horticulture and Forestry Nauni, Solan-173230, HP, India
S. V. Bhardwaj *
Department of Biotechnology Dr. Y S Parmar University of Horticulture and Forestry Nauni, Solan-173230, HP, India
A. K. Mangal
CRI (Ayurveda) Block-CN, Sector-V, Saltlake, Kolkata-700091
*Author to whom correspondence should be addressed.
Abstract
An ecofriendly approach to tackle Carnation Latent Virus in carnation cv Scania has been standardized. Addition of four different concentrations viz. 1.0, 2.5, 5.0 and 7.5 mg/l of acetone extracts obtained from fruit and root fractions of Asparagus adscendens Roxb. in multiplication medium of carnation has shown that inhibitors are able to decrease the concentration of Carnation Latent Virus in the infected plants. Whereas the fruit fractions of A. adscendens Roxb. could eliminate the virus at 7.5 mg/l, the root fractions were found to be effective at 5.0 and 7.5 mg/l. This is the first report of inhibition / elimination of a plant virus by a virus inhibitor of plant origin under in vitro conditions.
Keywords: Asparagus adscendens, Carnation, Enzyme linked immunosorbent assay, Virus