Efficient In Vitro Clonal Propagation of Rauwolfia serpentina L.: An Important Medicinal Plant
Sharad Tiwari *
Plant Biotechnology Laboratory, Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482 004, MP, India
Pankaj Shah
Plant Biotechnology Laboratory, Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482 004, MP, India
Kanchan Singh
Plant Biotechnology Laboratory, Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482 004, MP, India
*Author to whom correspondence should be addressed.
Abstract
Nodal sections comprising of axillary buds of Rauwolfia serpentina were cultured to test their growth performance and regeneration potential on three different basal nutrient medium viz. Murashige and Skoog (MS), Gamborg�s (B5) and White�s (Wh) medium without growth hormones and with 3.0 mg l-1 6-BAP and 0.5 mg l-1 a-NAA denoted as MSBN, B5�BN and WhBN, respectively constituting overall six shoot induction media. Maximum multiple shoot regeneration was observed on B5�BN as compared to other five media. On MSBN medium the regenerated shoots attained maximum length however, the callus formation was less than that on B5BN. Maximum shoots with optimal nodes were obtained on medium B5BN. However, after 30 days of initial culture the shoot length was highest on MSBN. Overall, four to five fold improvement was observed for multiple shoot production In vitro with B5BN medium and two to three fold enhancement on MSBN as compared to WhBN. In vitro rooting was obtained efficiently prior to transplanting.
Keywords: In vitro rooting, Medicinal plant, Multiple shoot induction, Nodal explants, Rauwolfia serpentina