AUXIN PRODUCTION FROM PHYTOPATHOGENIC ORGANISMS
KIRAN S. CHUDASAMA
Department of Biosciences, Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering, Saurashtra University, Rajkot–360 005, Gujarat, India
VRINDA S. THAKER *
Department of Biosciences, Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering, Saurashtra University, Rajkot–360 005, Gujarat, India
*Author to whom correspondence should be addressed.
Abstract
Bacteria as plant pathogen are known to attack various crops of economic importance. Many of disease symptoms caused by phytopathogenic microbes appear to reflect the changes in plant hormones levels. In the present study, Xanthomonas campestris, Pantoea agglomerans, Bacillus subtilis, Lysinibacillus fusiformis, Lysinibacillus sphaericus, Ochrobactrum anthropi and Erwinia chrysanthemi were isolated from the different infected plant materials and identified by 16s rDNA sequencing. They were characterized by IAA (Indole Acetic Acid) production, a phytohormone known to play important role in plant-microbe interactions. Conditions for IAA production was optimized in a nutrient medium containing tryptophan and estimated by Salkowaski reagent. It was observed that Erwinia chrysanthemi produced maximum IAA concentration compared to other bacteria.
Keywords: Plant pathogens, 16s rDNA, auxin