Epigenesis and High Frequency Plant Regeneration from Hypocotyl Explants of Soybean (Glycine max L. Merrill)
Manoj Tripathi
Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics Jawaharlal Nehru Agricultural University, Jabalpur-482004, MP, India
Sharad Tiwari *
Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics Jawaharlal Nehru Agricultural University, Jabalpur-482004, MP, India
*Author to whom correspondence should be addressed.
Abstract
High frequency somatic embryo induction was achieved in Glycine hypocotyls depending upon genotype and the explant inoculation medium. Plant regeneration efficiency was in excess of 85% following plural shoot initiation in genotype JS 90-41 when the in vitro inoculation background was a combination of 0.5 mg l-1 NAA and 3.0 mg l-1 BAP and contained MS macro - and micro - nutrients, vitamins and agar. Phenotypically normal plants were regenerated from the hypocotyl explants.
Keywords: Callus, Glycine max, Hypocotyl, In vitro morphogenesis, Soybean