In Vitro Plant Regeneration from Nodal and Leaf Explants of Solanum surattense Burm. F. A Medicinal Plant
Y. N. Seetharam *
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
G. Jyothishwaran
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
H. Sujeeth
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
Aravind Barad
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
G. Sharanabasappa
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
T. Sangeetha Panchal
Department of Botany, Gulbarga University, Gulbarga-585 106, Karnataka, India
*Author to whom correspondence should be addressed.
Abstract
An efficient method was developed for in vitro propagation Solanum surattense Burm. f. Multiple shoot formation was noticed on Murashige and Skoog�s medium supplemented with 2 mg/l 6-benzylaminopurine and 1 mg/l kinetin from nodal explants and 4 mg/l 6-benzylaminopurine and 1 mg/l kinetin from leaf explants. Shoot elongation was occurred on MS medium supplemented with 1 mg/l 6-benzylaminopurine and 1 mg/l gibberellic acid. Efficient rooting was achieved on � strength MS medium supplemented with 1 mg/1 indole3-butyric acid and 0.5 mg/l phloroglucinol. The regenerated plantlets were acclimatized and successfully transferred to the field condition.
Keywords: Leaf explants, Multiple shoots, Nodal explants, Shoot elongation, Solanum surattense Abbreviations: BAP - 6-Benzylaminopurine, GA3-gibberellic acid, IBA - indole3-butyric acid, Kn-Kinetin, MS-Murashige and Skoog�s medium, PG- Pholoroglucinol