In Vitro Callus Induction and Plantlet Regeneration from Indian Cotton Cultivars
Sucheta Tripathy *
Center for Plant Molecular Biology, Department of Genetics, Osmania University, Hyderabad-500 007, A.P., INDIA
G. M. Reddy
Center for Plant Molecular Biology, Department of Genetics, Osmania University, Hyderabad-500 007, A.P., INDIA
*Author to whom correspondence should be addressed.
Abstract
Efficient plant regeneration from callus cultures of Indian cotton cultivars is reported here. Ten Indian cotton cultivars namely NCS3, NA920, NHH390, NA1325, PMC, LRA, MCU5, DCH32, Srisailam and Anjali were used for the present experiment. Different explants such as epicotyl, hypocotyl, cotyledon, leaf, stem and in vitro grown leaf were used for callus induction and plant regeneration. Cotyledon was found to be best explant for in vitro study. From the ten cultivars studied, six cultivars viz. NCS3, NA920, NA 1325, Srisailam, LRA and PMC were responded well for plant regeneration. Among the different basal media (MS, B5, LS and Whites) and hormones (NAA, BA, KN, TDZ, Zeatin) tried in different combinations only MS media supplemented with 2 mg/l BA and 2 mg/l IAA responded best for plant regeneration. Maximum regeneration frequency obtained was 8.2% with NA920 cultivar. The protocol developed here can be used for genetic transformation experiments of Indian Cotton cultivars.
Keywords: Callus initiation, Genotype, Indian cotton cultivars, Plant regeneration